e3 ligase reaction buffer (Boston Biochem)
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e3 ligase reaction buffer
E3 Ligase Reaction Buffer, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e3+ligase+reaction+buffer/e3+ligase+reaction+buffer/pm40408472-312-81-85
Average 90 stars, based on 1 article reviews
E3 Ligase Reaction Buffer, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/e3+ligase+reaction+buffer/e3+ligase+reaction+buffer/pm40408472-312-81-85
Average 90 stars, based on 1 article reviews
e3 ligase reaction buffer - by Bioz Stars,
2026-10
90/100 stars
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Ubiquitin Proteomics:Article Title: A heterotypic assembly mechanism regulates CHIP E3 ligase activity. Article Snippet: .. The reactions were run at 30°C for 90 min using 60 lM ubiquitin and its variants (Boston Biochem) in the presence of 100 nM E1 (UBE1, Boston Biochem), 0.6 lM E2 (Boston Biochem), E3 ligase (CHN-1 and variants or UFD-2 and variants), Article Title: Structural basis for substrate recruitment by AMBRA1 E3 ligase receptor Article Snippet: Imaging was performed using a Zeiss LSM 900 confocal microscope with a Plan-Apochromat 63×/1.40 oil objective. .. The ubiquitination assays were performed in a 25 μl reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2-627), 0.3 μM purified AMBRA1-DDB1-Cullin4-RBX1 complex, 20 μM HA-ubiquitin (Boston Biochem U-110), 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in Article Title: Mechanism of D-type cyclin recognition by the AMBRA1 E3 ligase receptor. Article Snippet: .. The ubiquitination assays were performed in a 25- μl reaction volume with the following components: 100 nM ubiquitin- like modifieractivating enzyme 1 (UBE1) (Boston Biochem, E- 304), 1.5 μM ubiquitin- conjugating enzyme H5C (UBCH5C) (Boston Biochem, E2- 627), 0.3 μM purified AMBRA1WD40- DDB1- Cullin4- RBX1 or AMBRA1FL- DDB1- Cullin4- RBX1 complex, 20 μM HA- ubiquitin (Boston Biochem, U- 110), 0.16 μM TSF–D- type cyclins–CDK4EE complex or GST–cyclin D1 C termini, and 10 mM magnesium- ATP solution (Boston Biochem, B- 20) in Article Title: A heterotypic assembly mechanism regulates CHIP E3 ligase activity Article Snippet: .. The reactions were run at 30°C for 90 min using 60 μM ubiquitin and its variants (Boston Biochem) in the presence of 100 nM E1 (UBE1, Boston Biochem), 0.6 μM E2 (Boston Biochem), E3 ligase (CHN‐1 and variants or UFD‐2 and variants), Article Title: Disease biomarkers and treatment methods related thereto Article Snippet: The beads were washed 3 times with IP lysis buffer (Pierce) and protein was eluted from the affinity gel with 250 μg/ml FLAG peptide (Sigma) after incubation for 30 min at 4° C. Protein lysates were then analyzed as described above. .. The beads were washed 3× in IP lysis buffer and 2× in Article Title: CHIP ubiquitin ligase is involved in the nucleolar stress management Article Snippet: .. The reactions were run at 37°C for 90 min using 60 μM Ubiquitin (Boston Biochem) in the presence of 100 nM E1 (UBE1, Boston Biochem), 0.6 μM E2 (Boston Biochem), Article Title: Structure of the DDB1-AMBRA1 E3 ligase receptor complex linked to cell cycle regulation Article Snippet: The cryo-EM density map has been deposited in the Electron Microscopy Data Bank under accession code EMD-37752 and the coordinates have been deposited in the Protein Data Bank under accession number 8WQR. .. The ubiquitination assays were performed in a 25 μL reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2-627), 0.3 μM purified AMBRA1-DDB1-Cullin4-RBX1 complex, 20 μM HA-ubiquitin (Boston Biochem U-110), 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in Article Title: Mechanism of D-type cyclins recognition by the AMBRA1 E3 ligase receptor Article Snippet: .. The ubiquitination assays were performed in a 25 μL reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2- 627), 0.3 μM purified AMBRA1 WD40 -DDB1-Cullin4-RBX1 or AMBRA1 FL -DDB1-Cullin4-RBX1 complex, 20 μM HA-ubiquitin (Boston Biochem U-110), 0.16 μM TSF-D-type cyclins-CDK4 EE complex or GST-cyclin D1 C-termini, and 10 mM MgATP solution (Boston Biochem B-20) in Purification:Article Title: Structural basis for substrate recruitment by AMBRA1 E3 ligase receptor Article Snippet: Imaging was performed using a Zeiss LSM 900 confocal microscope with a Plan-Apochromat 63×/1.40 oil objective. .. The ubiquitination assays were performed in a 25 μl reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2-627), 0.3 μM purified AMBRA1-DDB1-Cullin4-RBX1 complex, 20 μM HA-ubiquitin (Boston Biochem U-110), 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in Article Title: Mechanism of D-type cyclin recognition by the AMBRA1 E3 ligase receptor. Article Snippet: .. The ubiquitination assays were performed in a 25- μl reaction volume with the following components: 100 nM ubiquitin- like modifieractivating enzyme 1 (UBE1) (Boston Biochem, E- 304), 1.5 μM ubiquitin- conjugating enzyme H5C (UBCH5C) (Boston Biochem, E2- 627), 0.3 μM purified AMBRA1WD40- DDB1- Cullin4- RBX1 or AMBRA1FL- DDB1- Cullin4- RBX1 complex, 20 μM HA- ubiquitin (Boston Biochem, U- 110), 0.16 μM TSF–D- type cyclins–CDK4EE complex or GST–cyclin D1 C termini, and 10 mM magnesium- ATP solution (Boston Biochem, B- 20) in Article Title: Structure of the DDB1-AMBRA1 E3 ligase receptor complex linked to cell cycle regulation Article Snippet: The cryo-EM density map has been deposited in the Electron Microscopy Data Bank under accession code EMD-37752 and the coordinates have been deposited in the Protein Data Bank under accession number 8WQR. .. The ubiquitination assays were performed in a 25 μL reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2-627), 0.3 μM purified AMBRA1-DDB1-Cullin4-RBX1 complex, 20 μM HA-ubiquitin (Boston Biochem U-110), 0.16 μM myc-Cyclin D1-CDK4 complex and 10 mM MgATP solution (Boston Biochem B-20) in Article Title: Mechanism of D-type cyclins recognition by the AMBRA1 E3 ligase receptor Article Snippet: .. The ubiquitination assays were performed in a 25 μL reaction volume with the following components: 100 nM UBE1 (Boston Biochem E-304), 1.5 μM UBCH5C (Boston Biochem E2- 627), 0.3 μM purified AMBRA1 WD40 -DDB1-Cullin4-RBX1 or AMBRA1 FL -DDB1-Cullin4-RBX1 complex, 20 μM HA-ubiquitin (Boston Biochem U-110), 0.16 μM TSF-D-type cyclins-CDK4 EE complex or GST-cyclin D1 C-termini, and 10 mM MgATP solution (Boston Biochem B-20) in Lysis:Article Title: Disease biomarkers and treatment methods related thereto Article Snippet: The beads were washed 3 times with IP lysis buffer (Pierce) and protein was eluted from the affinity gel with 250 μg/ml FLAG peptide (Sigma) after incubation for 30 min at 4° C. Protein lysates were then analyzed as described above. .. The beads were washed 3× in IP lysis buffer and 2× in |